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Genetic variation of barley germplasm from Turkey assessed by chloroplast microsatellite markers

Abstract

Hülya SİPAHİ, Hasan MEYDAN and Kürşad ÖZBEK

Five chloroplast DNA microsatellite loci were tested to analyze cytoplasmic variation in a set of 94 barley accessions; 23 Hordeum vulgare ssp. spontaneum (wild) from Southeastern Anatolia, 47 Hordeum vulgare ssp.vulgare landraces and 24 Turkish barley cultivars. Fifteen (15) haplotypes were detected. Within wild barley, two of the five loci were polymorphic with two or three alleles and four haplotypes were found, giving a gene diversity value of 0.31. One of these haplotypes was found in 13 Turkish cultivars. Two loci were polymorphic in the landraces, giving three haplotypes and a gene diversity value of 0.33. One of haplotypes found in landraces was observed in a Turkish cultivar. All loci were polymorphic in 24 Turkish cultivars and the numbers of alleles per locus ranged from two to three alleles, with a gene diversity value of 0.61. Of the six haplotypes detected in Turkish cultivars, three were unique to three cultivars. The chloroplast microsatellite loci were found multiallelic and variable within H. vulgare ssp. vulgare and H. vulgare ssp. spontaneum intraspesific accessions and useful to compare degrees of genetic variation between accessions. The results improved the knowledge in genetic variation of CRIFC seed genebank’s barley accessions and could be informative for broadening the genetic base of barley.

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