Ginsenoside retinoblastoma 1 (Rb1) suppresses NO production and inducible nitric oxide synthase (iNOS) expression by inhibiting nuclear factor κB (NF-κB) activation in SW1353 chondrosarcoma cells

Abstract

Ping Jia, Gang Chen, Rong-Heng Li, Xiao-Feng Rong, Guo-Qing Zhou and Yu Zhong

Ginseng (Panax ginseng C.A. Mey) is commonly used to treat osteoarthritis (OA) in Chinese traditional medicine (TCM). In this study, we investigated whether ginsenoside retinoblastoma 1 (Rb1), an active component of ginseng, could regulate NO production in chondrocytes and its potential mechanisms of action. SW1353 cells were stimulated with IL-1β in the presence of different concentrations of ginsenoside Rb1. NO concentration was assessed by the Griess reaction. Expression of iNOS, degradation of I Bα and nuclear translocation of NF-κB p65 were determined by Western blot. DNA binding activity of NF-κB complex was evaluated with Trans AM™ kit for p65. We found that ginsenoside Rb1 significantly decreased the NO production and iNOS protein expression in a concentrationdependent manner. Ginsenoside Rb1 markedly decreased the IκBα degradation and nuclear p65 levels, as well as inhibited the DNA binding activity of NF-κB complex. These results suggest that ginsenoside Rb1 inhibits IL-1β-induced NO production through downregulation of NF-κB-dependent iNOS expression in chondrocytes, and reveals potential mechanisms explaining the benefits of ginseng for OA treatment in TCM.

PDF

Share this article

tempobet

tempobet giriş

tempobet giriş

bursa escort

tipobet e yeni giriş süpertotobet yeni giriş süperbahis 940 tipobet imajbet giriş yap imajbet giriş yap