Effect of vitamin AD3E supplementation for haemorrhagic septicaemia vaccine in laboratory mice


Priyantha M. A. R., Siriwardhana B. A. M. P., N. Liyanagunawardana and A. A. Vipulasiri

Haemorrhagic septicemia is a fatal disease in tropical countries and annual vaccination is carried out in endemic areas. It is a killed vaccine with mineral oil as the adjuvant. The protection level of animal against infectious agent can be improved by the maintenance of proper nutrient status and vitamin supplementation. This method is followed by many vaccines both in animal and human medicine to enhance both cell mediated and humoral immunity. The objective of this study was to evaluate the logarithmic protection values of haemorrhagic septicaemia (HS) vaccine by vitamin AD3E supplementation in laboratory mice model. Active mouse protection test was performed by Reed and Muench’s method to calculate the logarithmic protection value in HS vaccine using mice model. The logarithmic protection value of non-supplemented group was 6.33 and supplemented groups were 6.73 and 6.75; and were found to be significant in treatment. It was concluded that the logarithmic protection level of vaccine can be significantly improved by the addition of vitamin AD3E in mice and presumption may be the same with livestock in field situation.

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