Development of made coral sponge as a haemostasis agent for acceleration of postoperative wound healing

Abstract

Anisa Irfani

Surgical injury is a trauma that is caused after doing surgery on the human body. The process of healing post-operative wounds requires a long time. There are phases of wound healing that must be passed until the wound heals. One important step that needs to be done is to control bleeding after surgery. Most bleeding controls that are used still have limitations, namely the nature that is not biodegradable so it cannot be absorbed by the body. General measures to stop bleeding generally use pressure or ligation or non-absorbable media. However, non-biodegradable agents in their use must be taken back after the wound heals which can trigger new bleeding. Calcium carbonate contains a high calcium element per unit weight. In addition calcium carbonate produces anti-inflammatory activity in acute and subacute inflammation, as well as a biocompatible and biodegradable material. Wound healing can be characterized by collagen density, the number of new blood vessels (angiogenesis), and the number of neutrophils. The purpose of this study was to determine whether artificial coral sponges containing calcium carbonate (CaCO3) have good haemostasis ability (characterized by an increase in the number of new blood vessels), a controlled level of inflammation (indicated by the number of neutrophils), and collagen density. Sixteen Sprague Dawley mice were divided into 4 groups with 4 mice in each group. Each rat was injured four times with a wound size of 1x1 cm². In three wounds different composition of the sponge was applied, namely one wound with 25% coral sponge, two wounds with 33% CaCO3 coral sponge, three wounds with gelatin-only sponge, and four wounds with gauze (control group). The tissue was taken on days 1, 4, 7, and 12 and histological preparations were stained with HE (Hematoxylin Eosin) and Mallory staining. After histology preparations were obtained, observations were made using a binocular microscope with a magnification of 4x100 objective lenses. Clinical observations showed that the wound with a CaCO3 coral sponge showed a more complete wound closure. (HISTORY RESULTS). Therefore overall, the results of this study indicate that there is an influence on the use of artificial coral sponges for accelerating postoperative wound healing.