Microbial degradation hydrogen sulphide by thiohalocapsa halophila isolated from industrial waste water treatment plant

Abstract

Hiral Borasiya and Maulin P Shah

Denaturing gradient electrophoresis gel was used to confirm the presence of Thiohalocapsa halophila and real-time PCR was used to quantify the level of these bacteria in the samples. The DGGE analysis showed a band for Thiohalocapsa halophila in all samples, with the strip being more leading in the test sample with H2S diffusion. It also showed that although a change has occurred in the diversity of the microbial population in the test sludge after almost eight weeks of H2S distribution, the structure of the microbial community the test and control was always similar. Polymerase chain reaction primers specific Thiohalocapsa halophila confirmed that 50% of isolates of both test and control bioreactors. Thiohalocapsa halophila the population has become more efficient in degrade broadcast H2S. This increase in efficiency was confirmed by a significant the increase in the number of isolates of the test mud with respect to those in the control mud when they were grown in a rich thiosulfate liquid medium.