Computational algorithm to assess the functionality of non-synonymous substitution and genetic relationship of DRA II gene of cattle, goat and sheep

Abstract

Dauda, A., Duwa, H. and Ebegbulem, V. N.

The study was conducted to examine amino acid substitution of Non-synonymous polymorphism and phylogenetic analysis of DRA II gene in cattle, goat and sheep. Twenty (20) nucleotide sequences comprising cattle (8), goat (5) and sheep (7). The following are the sequence accession numbers; AAI51472, AAI02117, AAI10003, AAI03364, AAI42073, AAI33510, AAI02565, AAI05234 (cattle), NP_001301117, XP_005696583, AEJ87223, AEJ87221, AEJ87220 (goat) and NP_001267646, XP_011955819, AEJ36201, AEJ36200, CAA77679, AEV89921, AAA16794 (sheep) were retrieved from gene bank (NCBI). Out of the Sixteen (16) amino acid substitution of cattle, (P10G, Q14V, V16I, R19A, K35Q, R38S, I40K and A41T) appeared beneficial while (F20C, K23L, A25C, N30I and H32P) appeared deleterious. Eighteen (18) amino acid substitution of goat (G10A, I13L, S18L, Q20G, W23G, E37S, Q33I, N39R, Q42G M47L and F72Q) appeared beneficial while (E45R, M60A, K63Q, T65M, L69G, and A76A) appeared deleterious. Sixteen amino acid substitutions of sheep (G10W, F12I, V15D, S18I, E21A, K26N and L38D) appeared beneficial while (V30S, F46D, D49K, D53V, H57P, L69G, E71L, F75A and F78K). The result revealed some intermingling between cattle and sheep which is in accordance with bovidea speciation