Samir Das, S V S Malik, Sameer Shrivastava, Pradeep Gandhale, Satish Kumar, Shabu Shoukat, Durga P Das, S B Barbuddheand D. B. Rawool
This is the first study ever carried out to develop and evaluate internalin C (InlC)-based serological assay for diagnosis of Listeria monocytogenes (LM) infection in goats using synthetic peptide as an antigen. Nine peptides representing major antigenic domains of InlC, a novel protein linked to the virulence of LM, were identified, analyzed, synthesized and employed in indirect enzyme-linked immunosorbent assay (ELISA) to evaluate their diagnostic potential using sera of goats experimentally inoculated with live and killed LM and from apparently healthy goats. Sera were screened by standardized indirect ELISA to reveal the antibodies against InlC (AInlC) as well as listeriolysin O (ALLO). Overall, the result revealed that the AInlC titres are lower than the ALLO titres. However, a fair correlation was observed between the titres of AInlC and ALLO in experimentally infected as well as apparently healthy goats. Based on the results obtained by both the ELISAs, it is suggested that InlC peptides alone may not serve as a suitable diagnostic antigen in indirect based ELISA for serodiagnosis of listeric infections. Further, there is need for identification, synthesis and evaluation of appropriate synthetic peptide(s) for essential virulence markers of listeriae whether existing or new marker, all need to be explored for developing an ultimate sensitive and specific rapid sero-diagnostics marker against listeriosis.
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